3.3 Differential expression analyses with DESeq2

3.3.1 VNN1 High VS Low

In the following subsections, samples were divided into two groups of same size (±1):

  • High: those with VNN1 signal above median
  • Low: those with VNN1 signal below median

Upregulated genes are those following VNN1 signal.

3.3.1.1 Projects subsets

3.3.1.1.1 TCGA-SARC

For the whole TCGA-SARC dataset, the differential expression analysis is done either considering only gender as covariate, or both gender and primary diagnosis.

3.3.1.1.1.1 Gender covariate
Volcano plot. 1072 significant genes according to Pvalue < 0.05 and |log2FoldChange| > 2 are highlighted in red and available in [this table](DT/resultsDeseq2Vnn1HighVsLowProjectIdTcgaSarcGenderCovKnitPostDeseq2TemplatePlotVolcanoDeseq2.html).

Figure 3.179: Volcano plot. 1072 significant genes according to Pvalue < 0.05 and |log2FoldChange| > 2 are highlighted in red and available in this table.

  • Enrichr results for all deregulated genes are available here
  • Enrichr results for upregulated genes are available here
  • Enrichr results for downregulated genes are available here
  • g:Profiler results for all deregulated genes are available here
  • g:Profiler results for all upregulated genes are available here
  • g:Profiler results for all downregulated genes are available here
  • g:Profiler results for comparison of the three queries above are available here